THE TRH-LIKE PEPTIDES IN RABBIT TESTIS ARE DIFFERENT FROM THE TRH-LIKE PEPTIDE IN THE PROSTATE

Citation
H. Linden et al., THE TRH-LIKE PEPTIDES IN RABBIT TESTIS ARE DIFFERENT FROM THE TRH-LIKE PEPTIDE IN THE PROSTATE, FEBS letters, 379(1), 1996, pp. 11-14
Citations number
21
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
00145793
Volume
379
Issue
1
Year of publication
1996
Pages
11 - 14
Database
ISI
SICI code
0014-5793(1996)379:1<11:TTPIRT>2.0.ZU;2-Q
Abstract
Human seminal fluid contains a number of tripeptide amides with simila r structures to thyrotropin releasing hormone (TRH), two of which have been identified as pGlu-Glu-Pro amide and pGlu-Phe-Pro amide, To dete rmine whether these peptides originate in the same tissues and have th e same molecular origin, TRH-immunoreactive peptides were extracted fr om the prostate and testis of the rabbit, purified by ion exchange chr omatography and HPLC, and identified by co-chromatography with H-3-lab elled marker peptides, In addition, trypsin digestion was used to rele ase TRH-like tripeptides from N-extended forms of these peptides, The sole TRH-like peptide in the prostate was shown to be pGlu-Glu-Pro ami de; it was not accompanied by a detectable amount of pGlu-Phe-Pro amid e, The prostate also appeared to contain a very small amount of N-exte nded forms of these peptides, In contrast to the prostate, the testis contained high concentrations of N-extended forms of pGlu-Phe-Pro amid e but essentially no tripeptide, The testis also contained N-extended forms of two other neutral TRH-like peptides which were less hydrophob ic than pGlu-Phe-Pro amide, Neither the prostate nor the testis contai ned a significant amount of TRH, The results show that in the rabbit t he TRH-like peptides pGlu-Glu-Pro amide and pGlu-Phe-Pro amide occur i n different tissues and appear to be formed from different precursors.