BILE-ACID ACTIVATION OF THE GADD153 PROMOTER AND OF P53-INDEPENDENT APOPTOSIS - RELEVANCE TO COLON-CANCER

Bile acids are strongly implicated in the etiology of colon cancer. Bi le acids also induce apoptosis, and this action may be a key to unders tanding their role in colon cancer. However the mechanism of bile acid induction of apoptosis is not known. We present evidence of bile acid activation of the gadd153 promoter (a promoter activated by DNA damag ing agents). We also show that bile acid induction of apoptosis is p53 -independent. In addition, bile salts were found to induce blebbing pr eceding the actual morphological onset of apoptosis, which indicates e arly cytoskeletal alterations.