ONLINE OPTIMAL-CONTROL OF INDUCED FOREIGN PROTEIN-PRODUCTION BY RECOMBINANT BACTERIA IN FED-BATCH REACTORS

Near-optimal control strategies for maximizing the production of induc ed foreign protein by recombinant bacteria, which were determined from optimal control simulation studies, were implemented using a two-lite r bioreactor. A proportional-integral feedback controller with anticip atory action and a self-tuning controller with anticipatory action wer e simulated and compared for regulation of glucose concentration along an optimal singular are production strategy. The proportional-integra l feedback control algorithm was implemented to suppress disturbances and model uncertainties while the anticipatory controller followed the computed singular are trajectories. Both simulation and experimental work proved that optimal control of the fed-batch system for induced f oreign protein production can be carried out on-line.