Jp. Andersen, DISSECTION OF THE FUNCTIONAL DOMAINS OF THE SARCOPLASMIC-RETICULUM CA2-ATPASE BY SITE-DIRECTED MUTAGENESIS(), Bioscience reports, 15(5), 1995, pp. 243-261
The results of site-directed mutagenesis studies of the sarcoplasmic r
eticulum Ca2+-ATPase are reviewed. More than 250 different point mutan
ts have been expressed in cell culture and analysed by a panel of func
tional assays. Thereby, 40-50 important amino acid residues have been
pinpointed, and the mutants have been assigned to functional classes:
the Ca2+-affinity mutants, the phosphorylation-negative mutants, the A
TP-affinity mutants, the E(1)P mutants, the E(2)P mutants, and the unc
oupled mutants. Moreover, regions important to the specific inhibition
by thapsigargin have been identified by analysis of Ca2+-ATPase/Na+,
K+-ATPase chimeric constructs.