2D NMR-STUDY ON 4-O-TETRAHYDROPYRANYLEPIISOPICROPODOPHYLLIN

A complete assignment of the two dimensional H-1 and C-13 NMR spectra of the title compound, which is a key intermediate for preparation of podophyllotoxin derivatives, is presented. The proton signals have bee n assigned from DQF- COSY, TOCSY, H-1-H-1 coupling patterns, and by th e comparisons of chemical shifs with those of similar podophyllum lign ans. Complete C-13 NMR assignments have been made from HMQC, HMBC and DEPT spectra. Further information on the stereochemistry of the molecu le was obtained from 2D NOESY and NOE-difference techniques.