EMODIN, A NATURALLY-OCCURRING ANTHRAQUINONE - ITS ISOLATION AND SPECTROPHOTOMETRIC DETERMINATION IN RUMEX CYPRIUS PLANT

Authors
ALNURI MA ZATAR NA ABUEID MA HANNOUN MA ALJONDI WJ HUSSEIN AI ALISHTAYEH MS
Citation
Ma. Alnuri et al., EMODIN, A NATURALLY-OCCURRING ANTHRAQUINONE - ITS ISOLATION AND SPECTROPHOTOMETRIC DETERMINATION IN RUMEX CYPRIUS PLANT, Spectroscopy letters, 29(8), 1996, pp. 1539-1543
Citations number
6
Categorie Soggetti
Spectroscopy
Journal title
Spectroscopy letters → ACNP
ISSN journal
00387010
Volume
29
Issue
8
Year of publication
1996
Pages
1539 - 1543
Database
ISI
SICI code
0038-7010(1996)29:8<1539:EANA-I>2.0.ZU;2-N
Abstract
A new method for isolation and specrophotometric determination of emod in is presented. Emodin was isolated by thin layer chromatography (tlc ) and column chromatography (cc) techniques, as an orange long crystal line substance. Emodin exhibits two absorption maxima, at 320 and 520 nm. Stability of the color and the effect of pH were studied. Beer's l aw is obeyed in the range 2-30 ppm. The method is applied to the deter mination of emodin in roots, stems, and leaves of Rumex cyprius plant. [GRAPHICS] on the stereochemistry of the molecule was obtained from 2 D NOESY and NOE-difference techniques.