POINT MUTATIONS IN THE KI-RAS2 GENE OF CODON-12 IN THE DUNNING R-3327PROSTATIC ADENOCARCINOMA SYSTEM

Detection of point mutations in the Dunning System with the Ki-ras2 ge ne in the first and second positions of codon 12 exon I, has been perf ormed using allele-specific oligonucleotide (ASO) primers. PCR generat ed 94bp templates and genomic DNA extracted from the Dunning cell line s AT-I, AT-3, Mat-Lu, and Mat-Lylu were tested. Our investigation reve aled that the first and second positions of codon 12 have undergone ei ther transitions or transversions from the wild type sequence (GG). Do rsal prostate (DP) solid tissues used as controls revealed the wild ty pe configuration as well as transversions at both positions, in additi on to a transition in the first position for Mat-Lu. The most aberrant of the Dunning lines (AT-3 and Mat-LyLu) collectively displayed seque nce changes (transitions and transversions) with no evidence of the wi ld type configuration. These findings are supportive of biometric stud ies (area, shape factor, and motility) along with adhesion and invasio n assays done in our laboratory in correlating genotypic and phenotypi c properties to metastatic potential. (C) 1996 Wiley-Liss, Inc.