DETERMINATION OF CHLOROANILINES IN ANTIBACTERIAL SOAPS USING CATION-EXCHANGE CHROMATOGRAPHY WITH UV DETECTION

A method is described for the rapid determination of 3,4-dichloroanili ne and 4-chloroaniline in antibacterial soaps. Limits of detection (si gnal-to-noise ratio = 3) were 0.5 mu g/g for 3,4-dichloroaniline and 2 mu g/g for 4-chloroaniline. Including day-to-day and system-to-system variability, recoveries from a placebo spiked with 50 mu g/g of each chloroaniline (n = 20) were 101.1 +/- 1.6% for 3,4-dichloroaniline and 102.9 +/- 7.6% for 4-chloroaniline. The method uses UV detection and is applicable to multiple formulations.