STUDIES OF HYDROGEN DEUTERIUM EXCHANGE OF MONODEUTERIOPHENOL IN FUSED-SILICA CAPILLARY COLUMNS USING GAS-CHROMATOGRAPHY MASS-SPECTROMETRY/

The hydrogen/deuterium (H/D) exchange process in fused-silica capillar y columns was extensively studied using two old and two new columns. T he compounds selected for this study were mixtures of phenol and monod euteriophenol with different compositions. The factors controlling and influencing this phenomenon, such as heating rate and flow-rate varia tion as a function of residence time on the internal column-surface, w ere also evaluated. Temperature programming and isothermal analysis we re performed for comparison. All studied factors proved that old fused -silica capillary columns exhibit sufficient activity to affect H/D ex change of monodeuteriophenol compounds, but the degree of exchange dep ends mainly on the position of ring deuteration. However, new columns were found to be less reactive toward H/D exchange and the percentage of exchange may be considered to be within the experimental error.