FRACTIONATION OF PEPTIDES IN PROTEASE DIGESTS OF PROTEINS BY PREPARATIVE ISOELECTRIC-FOCUSING IN THE ABSENCE OF ADDED AMPHOLYTE - A BIOCOMPATIBLE AND LOW-COST APPROACH REFERRED TO AS AUTOFOCUSING
M. Yata et al., FRACTIONATION OF PEPTIDES IN PROTEASE DIGESTS OF PROTEINS BY PREPARATIVE ISOELECTRIC-FOCUSING IN THE ABSENCE OF ADDED AMPHOLYTE - A BIOCOMPATIBLE AND LOW-COST APPROACH REFERRED TO AS AUTOFOCUSING, Journal of agricultural and food chemistry, 44(1), 1996, pp. 76-79
Peptides in the protease digests of some acidic and basic proteins in
the range of 0.1-1.0% (w/v) could be fractionated with high reproducib
ility by a preparative isoelectric focusing on the basis of the amphot
eric nature of samples in the absence of added carrier ampholytes. Enr
ichment of peptides of up to 15-fold could be achieved by the present
method. This technique, referred to as autofocusing, did not require o
r yield any toxic solvents or solutes; thus, the present approach can
be a low-cost and biocompatible method for peptide fractionation.