SEQUENCES OF CDNA CLONES ENCODING ALPHA-ACTIN OF CARP AND GOLDFISH SKELETAL-MUSCLES

We have isolated cDNA clones encoding skeletal muscle alpha-actins of carp Cyprinus carpio and goldfish Carassius auratus. The complete nucl eotide (nt) sequences of cDNA clones and their deduced amino acid sequ ences were determined. The coding region of cDNA clones contained 1131 nt for both carp and goldfish, while their 3' untraslated regions sho wed polyadenylation signals. When the two cDNA nucleotide sequences we re compared in the overlapping 1257 nt corresponding to the coordinate from -36 to 1221, 35 nt substitutions were observed. Among these, 21 substitutions occurred between T and C, while 25 of total 28 nt substi tutions in the coding region were located at the third base. Totally 3 77 amino acids were deduced from the coding region of both carp and go ldfish, although it was predicted that the two N-terminal amino acid r esidues, Met followed by Cys, would be processed after translation as the cases of other alpha-actins so far reported. Amino acid sequence d ifferences between carp and goldfish mature alpha-actins were Asp/Glu- 3 and Thr/Val-5.