The differences in water suppressed VOSY of the brain between a live a
nd dead mouse have been observed in experiments. In the water suppress
ed VOSY of the dead mouse brain some spectral lines near the water sig
nal have disappeared but these spectral lines do exist in the water su
ppressed VOSY of the live mouse brain. The approach used to obtain the
water suppressed VOSY of the mouse brain is termed phase-shift presat
uration, whose water suppression factor, tested by a phantom of an aqu
eous solution of ethanol, is greater than 1000.