INHERITANCE OF RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS IN ANASTREPHA-SUSPENSA (DIPTERA, TEPHRITIDAE)

Two full-sibling families of the Caribbean fruit By Anastrepha suspens a (Loew), were produced by controlled mating from the USDA-ARS colonie s located at Miami, FL. One family was produced from a colony populati on reared on a corncob diet as larvae. The other family was produced f rom a colony population reared on an agar diet as larvae. Colonies ori ginated from a common source but each had been maintained as an isolat ed population for > 800 generations. DNA was extracted from both paren ts and 36 full-sibling progeny from both families. Sixty-three random amplified polymorphic DNA (EWPD) primers were screened using DNA from the 4 parental flies and 97% (61) produced amplification products. Six polymorphic primers were selected for the analysis. These 6 primers a mplified 127 bands in both the corncob and the agar flies; of these, 8 7 and 77 bands were analyzed in the corncob and agar flies, respective ly. In the corncob flies, 55.2% of the analyzed loci were polymorphic: 49.4% were polymorphic in the agar flies. Both 1:1 and 3:1 segregatio n ratios of polymorphic loci were observed with 24 and 28 loci segrega ting 1:1, and 13 and 9 loci segregating 3:1 in the corncob and agar fl ies, respectively. Most RAPD primers generated polymorphic bands that segregated as expected. This indicated that polymorphic RAPD bands are useful as genetic markers in Caribbean fruit By. The markers are pote ntially useful for host and geographic population studies as they rela te to quarantine issues.