We have cloned and sequenced the human B1 bradykinin receptor gene (BD
KRB1), which contains an uninterrupted coding exon. A putative promote
r was identified by linking various lengths of the 5'-flanking region
of the B1 receptor gene coding sequence to a CAT reporter and assaying
for CAT activity. Deletion analysis showed that a 300-kp fragment in
the promoter region is sufficient to direct the synthesis of the repor
ter and that an enhancer-like element is present between -1842 and -81
2. A genomic Southern blot using the B1 cDNA revealed that: the recept
or is encoded by a single-copy gene. The gene is located on chromosome
14q32.1-q32.2, in close proximity to the B2 receptor gene. Northern b
lot analysis identified a 1.7- to 1.8-kb mature mRNA transcript of the
B1 receptor gene in the kidney and pancreas. A widespread tissue dist
ribution of the B1 gene expression was identified by RT-PCR-Southern b
lot analysis using specific oligonucleotide probes. (C) 1996 Academic
Press, Inc.