GENOMIC DNA-SEQUENCE, EXPRESSION, AND CHROMOSOMAL LOCALIZATION OF THEHUMAN B1 BRADYKININ RECEPTOR GENE BDKRB1

We have cloned and sequenced the human B1 bradykinin receptor gene (BD KRB1), which contains an uninterrupted coding exon. A putative promote r was identified by linking various lengths of the 5'-flanking region of the B1 receptor gene coding sequence to a CAT reporter and assaying for CAT activity. Deletion analysis showed that a 300-kp fragment in the promoter region is sufficient to direct the synthesis of the repor ter and that an enhancer-like element is present between -1842 and -81 2. A genomic Southern blot using the B1 cDNA revealed that: the recept or is encoded by a single-copy gene. The gene is located on chromosome 14q32.1-q32.2, in close proximity to the B2 receptor gene. Northern b lot analysis identified a 1.7- to 1.8-kb mature mRNA transcript of the B1 receptor gene in the kidney and pancreas. A widespread tissue dist ribution of the B1 gene expression was identified by RT-PCR-Southern b lot analysis using specific oligonucleotide probes. (C) 1996 Academic Press, Inc.