CHROMOSOMAL ASSIGNMENT OF 79 CDNAS FROM A RANGE OF HUMAN TISSUES

Single-pass DNA sequencing of cDNAs selected at random from a human mi xed tissue cDNA library have generated a series of more than 2000 expr essed sequence tags. One hundred twenty-eight unique cDNA fragments wi th little or no known protein or nucleic acid homologies have been sel ected for further analysis. Oligonucleotide primer pairs have been des igned from the cDNAs and used in PCR amplification in combination with genomic DNA from a panel of monochromosomal somatic cell hybrids. Thi s has allowed us to assign 70 of these transcribed genes to a single c hromosome, and a further 9 have been located on two or three chromosom es. Additionally, 3 cDNAs contain short tandem repeats that may allow them to be further localized by linkage analysis. (C) 1996 Academic Pr ess, Inc.