The possibility of using different strains of parotitis virus (Enders,
L-3, Jeryl-Leen) as antigens for enzyme immunoassay (EIA) to titer an
tibodies in human and animal blood sera is analyzed. Methods for prepa
ration and purification of antigen on the basis of the said parotitis
virus strains have been developed. Conditions of EIA were optimized. T
he sensitivity and specificity of EIA and hemagglutination inhibition
test were compared.