TETRAHYMENA-THERMOPHILA - ANALYSIS OF PHOSPHOLIPIDS AND PHOSPHONOLIPIDS BY HIGH-FIELD H-1-NMR

The phospholipids of control and lipid-modified Tetrahymena thermophil a were identified and quantified, using 1-D and 2-D COSY proton NMR sp ectroscopy on intact lipids, before and after HPLC separation. The res ults are comparable to those obtained using classical lipid analytical techniques. The results indicate that the study of enzyme pathways an d other metabolic processes involving phospholipids in Tetrahymena and related protozoa can be carried out using proton NMR spectroscopy as the investigating technique.