RELATIVE RESISTANCE OF THE HAMSTER TO AORTIC ATHEROSCLEROSIS IN SPITEOF PROLONGED VITAMIN-E-DEFICIENCY AND DIETARY HYPERCHOLESTEROLEMIA - PUTATIVE EFFECT OF INCREASED HDL
O. Stein et al., RELATIVE RESISTANCE OF THE HAMSTER TO AORTIC ATHEROSCLEROSIS IN SPITEOF PROLONGED VITAMIN-E-DEFICIENCY AND DIETARY HYPERCHOLESTEROLEMIA - PUTATIVE EFFECT OF INCREASED HDL, Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1299(2), 1996, pp. 216-222
Male golden hamsters were rendered hypercholesterolemic by feeding die
ts enriched with cholesterol and fat, In the first series of experimen
ts, 5% butter and 1% cholesterol were added to a chow diet and plasma
cholesterol levels were maintained at 350-390 mg/dl over the entire ex
perimental period. Groups of hamsters and their age controls consuming
the chow diet, were killed after 7, 15 and 20 months when the aorta w
as examined for atherosclerosis by determination of cholesterol mass.
In the controls, aortic total cholesterol (TC) increased with age by 2
8% and esterified cholesterol increased to 11% of TC. In the hyperchol
esterolemic animals aortic TC was only 28% higher than in the controls
and cholesteryl ester was also 11.5% of TC, In the second series, one
group of hamsters were fed a semi-purified diet deficient in vitamin
E, containing 1% cholesterol and 10% lard; a second group received the
same diet, but supplemented with vitamin E. Controls consumed local c
how. After 7 months on the vitamin E deficient diet plasma alpha-tocop
herol was 0.05 mg/l, in those supplemented with vitamin E it was 20 mg
/l, while in the controls it was 3.3 mg/l. Plasma thiobarbituric acid
reactive substances (TEARS) were higher in the vitamin E deficient gro
up and then was a greater propensity of lipoproteins (d < 1.063 g/ml)
to peroxidation in vitro than in the vitamin E supplemented group. Pla
sma cholesterol was 366 mg/dl in the vitamin E deficient, 336 mg/dl in
the vitamin E supplemented group, and 64 mg/dl in controls. Aortic ch
olesterol was 79.1 in vitamin E supplemented and 84.4 mu g/10 mg dry w
eight in vitamin E deficient hamsters. In both series of experiments,
HDL amounted to 36-41% of plasma TC in the hypercholesterolemic animal
s and 59-62% in the controls. In conclusion: the hamster appears to be
quite resistant to atherosclerosis in face of sustained hypercholeste
rolemia, even in the presence of increased peroxidative stress caused
by vitamin E deficiency. This relative resistance could be related to
commensurate increase in plasma HDL which was observed in both series
of experiments. Since vitamin E deficiency did not enhance aortic chol
esteryl ester deposition, the protective effect of HDL seems to be rel
ated to its role in reverse cholesterol transport, rather than in prev
ention of peroxidation.