TISSUE-CULTURE AND TRANSFORMATION OF OENOTHERA-BIENNIS

Citation
D. Pavingerova et al., TISSUE-CULTURE AND TRANSFORMATION OF OENOTHERA-BIENNIS, Biologia plantarum, 38(1), 1996, pp. 27-32
Citations number
10
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00063134
Volume
38
Issue
1
Year of publication
1996
Pages
27 - 32
Database
ISI
SICI code
0006-3134(1996)38:1<27:TATOO>2.0.ZU;2-G
Abstract
Five cultivars of Oenothera biennis have been tested for callogenesis and organogenesis on different media. The cultivar CV3 has been transf ormed by Agrobacterium tumefaciens strain which introduces into the pl ant genome kanamycin resistance gene and the T-DNA ipt gene which caus es increased levels of cytokinins. Transformed tissues showed elevated levels of cytokinins and grew as teratomas forming clumps of short, b ranched shoots with small modified leaves. Roots appeared rarely in la ter subcultivations of some teratomous clones.