TISSUE-CULTURE AND TRANSFORMATION OF OENOTHERA-BIENNIS

Five cultivars of Oenothera biennis have been tested for callogenesis and organogenesis on different media. The cultivar CV3 has been transf ormed by Agrobacterium tumefaciens strain which introduces into the pl ant genome kanamycin resistance gene and the T-DNA ipt gene which caus es increased levels of cytokinins. Transformed tissues showed elevated levels of cytokinins and grew as teratomas forming clumps of short, b ranched shoots with small modified leaves. Roots appeared rarely in la ter subcultivations of some teratomous clones.