BETA-GALACTOSIDASE IN IMMOBILIZED CELLS OF PAPAVER-SOMNIFERUM

Authors
STANO J NEMEC P KAKONIOVA D KOVACS P LISKOVA D MICIETA K
Citation
J. Stano et al., BETA-GALACTOSIDASE IN IMMOBILIZED CELLS OF PAPAVER-SOMNIFERUM, Biologia plantarum, 38(1), 1996, pp. 123-127
Citations number
14
Categorie Soggetti
Plant Sciences
Journal title
Biologia plantarum → ACNP
ISSN journal
00063134
Volume
38
Issue
1
Year of publication
1996
Pages
123 - 127
Database
ISI
SICI code
0006-3134(1996)38:1<123:BIICOP>2.0.ZU;2-J
Abstract
Cell suspension culture of poppy was permeabilized by Tween 80 and imm obilized by glutaraldehyde. beta-Galactosidase showed optimum pH 4.8 a nd temperature of 50 degrees C. The enzyme hydrolysis was linear durin g 3 h reaching 68% conversion. The cells characterized by high beta-ga lactosidase activity and stability in long-term storage showed conveni ent physico-mechanical properties.