ETHANOLIC FERMENTATION OF XYLOSE WITH SACCHAROMYCES-CEREVISIAE HARBORING THE THERMUS-THERMOPHILUS XYLA GENE, WHICH EXPRESSES AN ACTIVE XYLOSE (GLUCOSE) ISOMERASE

Authors
WALFRIDSSON M BAO XM ANDERLUND M LILIUS G BULOW L HAHNHAGERDAL B
Citation
M. Walfridsson et al., ETHANOLIC FERMENTATION OF XYLOSE WITH SACCHAROMYCES-CEREVISIAE HARBORING THE THERMUS-THERMOPHILUS XYLA GENE, WHICH EXPRESSES AN ACTIVE XYLOSE (GLUCOSE) ISOMERASE, Applied and environmental microbiology, 62(12), 1996, pp. 4648-4651
Citations number
25
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
62
Issue
12
Year of publication
1996
Pages
4648 - 4651
Database
ISI
SICI code
0099-2240(1996)62:12<4648:EFOXWS>2.0.ZU;2-X
Abstract
The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the contro l of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees c with a specific activity of 1.0 U mg(-1). A new functional metabolic pathway in S. cerevisiae with etha nol formation during oxygen-limited xylose fermentation was demonstrat ed. Xylitol and acetic acid were also formed during the fermentation.