F. Barbato et al., RETENTION BEHAVIOR OF ANTIEMETIC SEROTONIN ANTAGONISTS IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Il Farmaco, 50(12), 1995, pp. 875-880
Granisetron, Ondansetron and Tropisetron, three 5-HT3 antagonists show
ing antiemetic activity, were analysed by HPLC on Lipophilic stationar
y phases. The addition of an amine or quaternary ammonium salt to the
eluents was a powerful tool in the analysis of these basic substances.
The influence on chromatographic parameters of pH, ionic strength and
various counter-ions in the aqueous phase as well as of different org
anic modifiers is discussed. Some of the proposed experimental conditi
ons allow a more strictly partition-based separation mechanism and can
produce chromatographic parameters suitable for structure-activity st
udies. These experimental conditions are also suitable for analysis of
the considered compounds in pharmaceutical dosage forms or in biologi
cal fluids.