Hs. Hyun et al., EFFECTS OF POSTOPERATIVE PERIODS ON GLUCOSE-ABSORPTION AND ELECTROLYTE SECRETION IN THE INTESTINAL LOOP OF SHEEP, Journal of veterinary medicine. Series A, 42(9), 1995, pp. 601-610
This study was designed to determine the effect of postoperative perio
ds on intestinal absorptive and secretory functions in the jejunal Thi
ry-Vella loop of sheep. The lumen was perfused with isotonic saline so
lution for 120 min at 1 ml/min. The secretory function was assessed by
measuring the secretory response of water and electrolytes to PGE, wh
ich was applied intraluminally at 100 mu M during the second 30 min pe
riod. To measure glucose absorption, 10 mM isotonic glucose solution w
ith its osmolality adjusted by adding NaCl was perfused. Measurements
were taken at 1 week intervals for 3 months after surgery. Under basal
conditions, water, sodium and chloride were absorbed, while potassium
and bicarbonate were secreted. These net basal movements did not chan
ge throughout. the 3 month period. PGE, reversed the net absorptions o
f water, sodium and chloride to the net secretions, and significantly
augmented bicarbonate secretion. Potassium secretion was not changed s
ignificantly by PGE,. These responses to PGE(2) were not affected by t
he postoperative periods. Glucose absorption decreased gradually for 8
weeks after construction of the jejunal loop with a significant negat
ive correlation to the postoperative period, and subsequently remained
at a steady state of nearly 65% of the first week. This decrease in g
lucose absorption was prevented by regular perfusion of glucose. The r
esults show that the Thiry-Vella loop of the ovine jejunum can be used
to measure secretory function for 3 months or more after its construc
tion, but it is essential to regularly perfuse the loop with glucose s
olution when this loop is used for absorptive experiments of glucose f
or a long time.