BINDING-PROPERTIES OF FIBRINOGEN RECEPTOR GPIIB-IIIA PURIFIED FROM HUMAN ERYTHROLEUKEMIA-CELLS

Large amounts (2.3 mg) of an inactive form of the glycoprotein GPIIb-I IIa were obtained in highly purified form from 7-liter cultures of hum an erythroleukemia (HEL) cells. The purified GPIIb-IIIa was converted to an activated form after its immobilization to 96-well plastic plate s. The binding of fibrinogen to the activated GPIIb-IIIa was investiga ted using 24 kinds of RGD peptides and showed that the IC50 values of these peptides correlated with those obtained with activated platelets . These findings indicated that the binding properties of the activate d GPIIb-IIIa from HEL cells are quite similar to those of the platelet s. (C) 1995 Academic Press, Inc.