AGROBACTERIUM-TUMEFACIENS-MEDIATED TRANSFORMATION OF VIGNA-MUNGO (L) HEPPER

Transformed Vigna mungo (blackgram) calli were obtained by cocultivati ng segments of primary leaves with Agrobacterium tumefaciens vir helpe r strains harbouring the binary vector pGA472 having kanamycin resista nce gene as plant transformation marker. Transformed calli were select ed on Murashige and Skoog medium supplemented with 50 mg/l kanamycin a nd 500 mg/l carbenicillin. Transformed calli were found to be resistan t to kanamycin up to 900 mg/l concentration. Expression of kanamycin r esistance gene in transformed calli was demonstrated by neomycin phosp hotransferase assay. Stable integration of transferred DNA into V. mun go genome was confirmed by Southern blot analysis.