RELATIONSHIP BETWEEN CALBINDIN-D28K LEVELS IN THE A-CELLS AND B-CELLSOF THE RAT ENDOCRINE PANCREAS AND THE SECRETION OF INSULIN AND GLUCAGON - INFLUENCE OF VITAMIN-D-3 DEFICIENCY AND 1,25-DIHYDROXYVITAMIN D-3

The pancreatic B cell is equipped with specific receptors for 1,25-dih ydroxyvitamin D-3 (1,25-(OH)(2)D-3) and contains vitamin D-dependent c alcium binding proteins (calbindin-D). Insulin secretion is impaired b y vitamin D deficiency and is restored by 1,25-(OH)(2)D-3 (concomitant ly with an improved calcium handling within B cells) but the effect of 1,25-(OH)(2)D-3 on the pancreatic B cell via calbindin-D is unclear. Therefore we examined the relationship between calbindin-D28K or calbi ndin-D9K and the activity of the endocrine pancreas in normal (N), fou r week vitamin D-deficient (- D) and one week 1,25-(OH)(2)D-3-replete (+ D) rats. Calbindin-D9K was not found in the pancreas, neither in th e islets nor in the exocrine part, of any of the groups of rats (N, - D, or + D). Surprisingly, total islet calbindin-D28K content was incre ased by vitamin D deficiency and partly restored by 1,25-(OH)(2)D-3. C albindin-D28K immunostaining was observed only on A and B cells in the endocrine part of the pancreas, the greatest staining being found in A cells. This difference in staining density was increased by vitamin D deficiency and decreased by 1,25-(OH)(2)D-3 treatment. In vitro, 1,2 5-(OH)(2)D-3 also produced a negative influence on calbindin-D28K stai ning in A cells, as demonstrated using pieces of pancreas incubated wi th the steroid for 2 h. No significant influence on labeling intensity of B cell calbindin-D28K could be shown. Plasma insulin and islet ins ulin release in response to 10 mM arginine stimulation were decreased in - D rats and enhanced in + D rats towards N values. In contrast, pl asma glucagon and the amount of glucagon secretion, stimulated in vitr o by 10 mM arginine of by low (1.7 mM) glucose concentration, was incr eased in - D rats and attenuated by 1,25-(OH)(2)D-3. Thus there appear s to be no relationship between the steady state level of B cell calbi ndin-D28K and the regulation of insulin secretion by 1,25-(OH)(2)D-3 i n vitamin D-deficient rats. However there is a correlation between A c ell calbindin-D28K and glucagon secretion, which are both negatively r egulated by 1,25-(OH)(2)D-3. The predominance of calbindin-D28K in A c ells raises the question as to how A and B cells interact and the role of calbindin-D28K in calcium handling.