LABORATORY TECHNIQUES IN DRUG DEVELOPMENT .6. PHARMACOKINETICS OF THEENANTIOMERS OF IFOSFAMIDE, 2-DECHLOROETHYLIFOSFAMIDE AND 3-DECHLOROETHYLIFOSFAMIDE IN PLASMA, URINE AND CEREBROSPINAL-FLUID

A chiral gas chromatographic method was used to determine the concentr ation and enantiomeric ratios of ifosfamide, 2- and 3-dechloroethylifo sfamide in plasma, urine and cerebrospinal fluid samples from 10 patie nts treated with ifosfamide. It appeared that some metabolic preferenc e exists for S-ifosfamide. However, this preference does not always le ad to a higher excretion of the metabolite isomers, S-2- and R-3-dechl oroethylifosfamide originating from S-ifosfamide. The differences in s tereospecific metabolism could not be correlated with age, sex, durati on of the infusion of ifosfamide dose.