DETECTION OF AMPHETAMINES IN URINE USING HEAD SPACE SOLID-PHASE MICROEXTRACTION AND CHEMICAL-IONIZATION SELECTED-ION MONITORING

An accurate, simple and rapid method for qualitative and quantitative analysis of amphetamine and methamphetamine in urine was developed usi ng head space-solid phase microextraction and gas chromatography-mass spectrometry/chemical ionization selected ion monitoring. A vial conta ining a urine sample potassium carbonate and pentadeuterated methamphe tamine which was used as an internal standard was heated at 80 degrees C for 20 min. The needle of a solid phase microextraction device was passed through the septum, and the extraction fiber in the needle was exposed for 5 min in the head space of the vial. The needle was remove d from the vial and inserted into the injection port of gas chromatogr aph or gas chromatograph/mass spectrometer. The compounds adsorbed on the fiber were detached by exposing the fiber in the injection port, a nd analyzed. The proposed method was more than 20 times more sensitive than the conventional head space method.