A. Wang et al., DIFFERENT EXPRESSION PATTERNS OF CYCLIN-A, CYCLIN-D1 AND CYCLIN-E IN HUMAN COLORECTAL-CANCER, Journal of cancer research and clinical oncology, 122(2), 1996, pp. 122-126
The expressions of cyclins A, D1 and E at the protein level were inves
tigated by Western blotting in human colorectal carcinomas and in adja
cent non-neoplastic colorectal mucosas. Cyclin E was higher in the can
cer tissue than in the non-neoplastic mucosa in 92% patients (35 out o
f 38 cases). However, the cyclin A expression of the mucosa was higher
than that of the cancer tissue in 63% (25 out of 40 cases) cases, and
only 4 (10%) cancers had higher cyclin A expression. Eleven cancers (
27%) demonstrated expression equivalent to that in the mucosa. Equal e
xpression of cyclin D1 in cancer and mucosal tissues was found in 51%
cases (20/39), lower expression of cyclin D1 by cancer tissues was dem
onstrated in 41% cases (16/39) and only three cancers showed higher ex
pression than the mucosa. Proliferating-cell nuclear antigen immunohis
tochemistry revealed that the labeling index of the cancer tissue was
43.5 +/- 8.3% while that of the mucosa was only 14.8 +/- 5.1%. These r
esults proved that colorectal cancers express high levels of cyclin E,
consistent with a high rate of cell proliferation, whereas most of su
ch cancer lose control of cyclin A and cyclin D1 expression.