Kj. Rao et T. Panda, RELEASE OF PERIPLASMIC PENICILLIN AMIDASE FROM ESCHERICHIA-COLI BY CHLOROFORM SHOCK, Bioprocess engineering, 14(2), 1996, pp. 101-103
Penicillin amidase is a periplasmic enzyme in Escherichia coli. Conven
tionally, the periplasmic enzymes are released into the medium by osmo
tic shock which is tedious involving a number of centrifugation steps.
The present communication deals with a simple technique for the relea
se of penicillin amidase by chloroform shock. Experimental findings sh
ow that the periplasmic penicillin amidase does not show any variation
by the chloroform treatment. This analysis was also extended to the E
. coli cells grown at various concentrations of phenylacetic acid, opt
imal concentration of phenylacetic acid plus glucose and lactic acid.