ENZYME-ACTIVITY MEASUREMENTS ON ISOLATED ORGANS OF BRACHIONUS-PLICATILIS (ROTIFERA)

A method is described by which the integument of Brachionus plicatilis , together with its intracellular lamina, is quickly dissolved before other parts or tissues of the animal are destroyed. After removing the integument several parts of the body can be separated and fractionate d in a more or less intact state by centrifugation in a Percoll gradie nt. The measurement of enzyme activities has indicated that this proce dure might provide a way of localizing enzymes within the rotifer body .