The efficiency of chitosan to reduce organic loads of effluents contai
ning various proportions of milk was investigated in laboratory scale.
Best results were obtained from substrates at a pH of 6.5. About 16 g
chitosan dissolved in 1 % acetic acid were required per kg organic ma
tter to obtain the optimum of precipitation. Higher concentration of m
ilk demanded a higher proportion of chitosan. In all levels of dilutio
n a residual amount of only 4 % of the original quantity of milk fat w
as left. The residues of protein to be found after treatment with chit
osan were proportional to the concentration of milk in the original su
bstrate. About 43 % of the original quantity of protein and 70 to 80 %
of lactose, as expected, could not be precipitated. About 50 % of the
original amount of organic matter could be removed from mixtures of m
ilk and water by the treatment with chitosan. No remarkable influence
of various degrees of deacetylation, reaching from 77 % to 94 %, and o
f ageing, from 1 to 8 weeks, of the solutions of chitosan were observe
d. In two samples, Chemical Oxygen Demand was reduced by treatment wit
h chitosan to approximately 40 % of the original level.