P-32 POSTLABELING OF BULKY HUMAN DNA-ADDUCTS ENRICHED BY DIFFERENT METHODS INCLUDING IMMUNOAFFINITY CHROMATOGRAPHY

DNA adducts in lymphocytes and granulocytes of men exposed occupationa lly and environmentally to high concentrations of aromatic compounds i n air were measured by the P-32-postlabeling method. Adducts in the sa me samples were characterised using nuclease P1 enrichment, butanol ex traction and immunoaffinity purification with an antiserum raised agai nst benzo[a]pyrene diol epoxide (BPDE). Only part of the adducts found in human samples were extracted by butanol. It also seemed, that only a small part of them belonged to the group of polycyclic aromatic hyd rocarbons (PAHs) recognised by the antibody. Relative content of hydro phobic adducts and those with a structure similar to PAHs was higher i n winter samples (when exposure to aromatic chemicals in air was highe r) in comparison to samples collected in summer.