DETERMINATION OF PHENYLMERCAPTURIC ACID IN URINE OF BENZENE-EXPOSED BDF-1 MICE

This paper describes a procedure for the identification of phenylmerca pturic acid in urine of benzene-exposed mice. Collected urine of benze ne exposed mice was adjusted to pH 7 and applied to an anion exchanger . After extraction with diethyl ether and evaporation to dryness, the sample was dissolved in aqueous phosphoric acid and injected into the HPLC. HPLC conditions included an ODS column and an eluent consisting of tetrabutylammoniumhydrogensulfate-methanol (75:25, v/v), the absorb ance wavelength was 255 nm. The detection limit of phenylmercapturic a cid was 3 mg/l in mouse urine.