MOUSE ANNEXIN-V CHROMOSOMAL LOCALIZATION, CDNA SEQUENCE CONSERVATION,AND MOLECULAR EVOLUTION

A full-length cDNA encoding mouse annexcin V (ANX5) was cloned, sequen ced, and utilized for chromosomal mapping. The gene Lies on mouse chro mosome 3 in close Linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counte rparts on human chromosome 4q. The open reading frame encoded a protei n of 319 amino acids (aa), with 92-96% identity to ANX5 in other speci es. Internal repeat 3 of mouse ANX5 exhibited the highest level of non conservative aa replacements with respect to other annexin subfamilies , but the greatest sequence conservation among ANX5 species members. T his region may thus contain features that distinguish ANX5 from other annexins in properties or function. Phylogenetic analysis and homology testing of AMX5 members indicated that the 34-kDa annexin from Torped o marmorata may also belong to this subfamily. Comparison of nine spec ies of ANX5 led to an estimation of the unit evolutionary mutation rat e at 1% aa replacements every 8 million years, comparable to other ann exins. (C) 1996 Academic Press, Inc.