A. Szeitz et al., SENSITIVE AND SELECTIVE ASSAY FOR FENTANYL USING GAS-CHROMATOGRAPHY WITH MASS-SELECTIVE DETECTION, Journal of chromatography B. Biomedical applications, 675(1), 1996, pp. 33-42
Citations number
23
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A modified gas chromatographic assay, using mass-selective detection,
has been developed for the quantitation of fentanyl in swine serum. Fe
ntanyl and sufentanil, the internal standard, were extracted using a s
ingle-step liquid-liquid extraction with dichloromethane. Sensitivity
and selectivity were improved by using electron-impact ionization (EI)
in the selected-ion monitoring (SIM) mode, where fentanyl and sufenta
nil were monitored using the fragment ions at m/z 245 and 289, respect
ively. The limit of quantitation (LOQ) is 0.05 ng/ml, using 1 ml of sa
mple, with a C.V. of 10.8% and a signal-to-noise ratio of 29. Standard
curves were linear (r(2) = 0.999) over the working range of 0.05-1.5
ng/ml, using 1/y(2) as a weighting factor. Recoveries averaged 69.8 +/
- 4.7%, 91.0 +/- 13.0% and 90.9 +/- 10.3% at serum concentrations of 1
.5, 0.5 and 0.1 ng/ml, respectively. Intra- and inter-day variances, w
ere <12% at 0.1 ng/ml, and <10% at concentrations of 0.5, 1 and 1.5 ng
/ml. Bias was 6.2% at the LOQ and less than or equal to 12.8% at every
other standard curve concentration. Applicability of the assay is dem
onstrated for the pharmacokinetic study of transdermally administered
fentanyl in a postoperative swine.