HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR GUANYLHYDRAZONE COMPOUNDS

A high-performance liquid chromatographic method has been developed fo r a series of aromatic guanylhydrazones that have demonstrated therape utic potential as anti-inflammatory agents. The compounds were separat ed using octadecyl or diisopropyloctyl reversed-phase columns, with an acetonitrile gradient in water containing heptane sulfonate, tetramet hylammonium chloride, and phosphoric acid. The method was used to reli ably quantify levels of analyte as low as 785 ng/ml, and the detector response was linear to at least 50 mu g/ml, using a 100 mu l injection volume. The assay system was used to determine the basic pharmacokine tics of a lead compound, CNI-1493, from serum concentrations following a single intravenous injection in rats.