MANUAL AND AUTOMATED (ROBOTIC) HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHYMETHODS FOR THE DETERMINATION OF MYCOPHENOLIC-ACID AND ITS GLUCURONIDE CONJUGATE IN HUMAN PLASMA
I. Tsina et al., MANUAL AND AUTOMATED (ROBOTIC) HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHYMETHODS FOR THE DETERMINATION OF MYCOPHENOLIC-ACID AND ITS GLUCURONIDE CONJUGATE IN HUMAN PLASMA, Journal of chromatography B. Biomedical applications, 675(1), 1996, pp. 119-129
Citations number
7
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A manual and an automated (Zymark PyTechnology robot) HPLC method for
simultaneous determination of plasma mycophenolic acid (MPA) and its g
lucuronide conjugate (MPAG) are described here. Both methods are repro
ducible and accurate, and both are equivalent in all respects, includi
ng quantification limits (MPA, 0.100 mu g/ml; MPAG, 4.00 mu g/ml), ran
ge (using 0.05-0.5 mi of plasma: MPA, 0.0500-20.0 mu g/aliquot; MPAG,
2.00-200 mu g/aliquot), precision, and accuracy. MPA and MPAG were sta
ble under the conditions used with both methods. Results from aliquots
of paired control samples, analyzed by the manual method over three y
ears at six analytical laboratories, showed excellent agreement in pre
cision and accuracy.