DIRECT DETERMINATION OF ZOLPIDEM AND ITS MAIN METABOLITES IN URINE USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION

Authors
HEMPEL G BLASCHKE G
Citation
G. Hempel et G. Blaschke, DIRECT DETERMINATION OF ZOLPIDEM AND ITS MAIN METABOLITES IN URINE USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION, Journal of chromatography B. Biomedical applications, 675(1), 1996, pp. 131-137
Citations number
6
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
675
Issue
1
Year of publication
1996
Pages
131 - 137
Database
ISI
SICI code
Abstract
Zolpidem is a new sleep inducer belonging to the imidazopyridine class . We wish to report a method for the determination of zolpidem and its main metabolites in urine without extraction using capillary electrop horesis with UV laser-induced fluorescence detection with a He-Cd lase r. A 10-nl sample of urine can be directly applied to the capillary. T he separation is carried out within 10 min, and the limit of detection is 2 ng/ml. This procedure is very simple and fast. No organic solven ts are necessary.