SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD WITH FLUORESCENCE DETECTION FOR MEASUREMENT OF VINORELBINE PLASMA-CONCENTRATIONS

A high-performance liquid chromatographic (HPLC) method with fluoresce nce detection for the determination of vinorelbine in plasma is descri bed. The technique was derived from that published by Debal for an ass ay of vinorelbine in cell culture medium. The modifications concern th e preparation procedure for plasma samples (a two-step liquid-liquid e xtraction from plasma is described), optimization of the mobile phase composition, and use of a single C-18 column. These changes resulted i n an improved sensitivity and reproducibility of the assay and led to its feasibility for clinical pharmacokinetic studies. The range of the assay is 2 to 1000 ng/ml.