ADJUSTMENT OF FLAVONOID ANALYSIS BY TLC U SING EXPERIMENTAL RESEARCH METHODOLOGY - STUDIES OF 2 LAMIACEA PLANTS

We develop the thin layer chromatography analysis of flavonoids extrac ted from two mediterranean plants. After selection of adsorbent with r egard to migration and spray reagent, we determined the best elution b lend by testing 11 samples cited in the literature. This solvent allow s a good chromatographic separation of flavonoids (aglycone+glucide). The aglycone identification, derived from acid hydrolysis of flavonoid s, is the first step in heterosid flavonoid studies. The variability o f aglycone polarities leads to difficult TLC separation. So we used th e experimental research methodology for optimising the solvent blend. The stimultaneous migration of standard is useful for the identificati on of two aglycones.