RADIOLYSIS STUDY OF THE MITOMYCIN C-DNA FORMATION MECHANISM OF ADDUCTS

Mitomycin C (MC) is an antibiotic used in the treatment of numerous ty pes of cancer. It is widely accepted that this agent must be reduced i n a one-electron way to undergo covalent bondings to DNA, RNA and vari ous proteins. Drug attachment investigations have been studied and cyt otoxic activity has been associated with the formation of the interstr and linked adducts. We report here radiolysis methods to understand wi th transient is responsible for the DNA cross-link adducts. These inve stigations provided three adducts with DNA and the alkylating intermed iate was characterized by its absorption spectrum and its formation ki netic of adducts.