GENOMIC MALE-STERILITY IN LETTUCE, A BASE-LINE FOR THE PRODUCTION OF F1 HYBRIDS

Citation
Is. Curtis et al., GENOMIC MALE-STERILITY IN LETTUCE, A BASE-LINE FOR THE PRODUCTION OF F1 HYBRIDS, PLANT SCI, 113(1), 1996, pp. 113-119
Citations number
18
Categorie Soggetti
Plant Sciences
Journal title
PLANT SCIENCE
ISSN journal
01689452 → ACNP
Volume
113
Issue
1
Year of publication
1996
Pages
113 - 119
Database
ISI
SICI code
0168-9452(1996)113:1<113:GMILAB>2.0.ZU;2-1
Abstract
Male sterile plants of lettuce (Lactuca sativa L.) cv. 'Lake Nyah' wer e produced using a genotype-independent transformation method employin g Agrobacterium tumefaciens with a binary vector. The latter carried t he neomycin phosphotransferase II gene controlled by the nopaline synt hase promoter and terminator sequences, enabling transformed shoots to be selected on medium containing kanamycin sulphate. In addition, exp ression of a pathogenesis-related glucanase gene linked to a tapetum-s pecific promoter, caused the dissolution of the callose wall of develo ping microspores in transgenic plants. All transformed plants were mal e sterile. Plants regenerated from uninoculated, excised cotyledons of 'Lake Nyah' exhibited normal microspore development and were fertile. These results suggest that callose degradation may cause male sterili ty and provides the opportunity for producing F1 hybrid seed in lettuc e.