EVIDENCE FOR NON-VACUOLAR LOCALIZATION OF ANTHOCYANOPLASTS (ANTHOCYANIN-CONTAINING VESICLES) IN SUSPENSION-CULTURED GRAPEVINE CELLS

Authors
CALDERON AA PEDRENO MA MUNOZ R BARCELO AR
Citation
Aa. Calderon et al., EVIDENCE FOR NON-VACUOLAR LOCALIZATION OF ANTHOCYANOPLASTS (ANTHOCYANIN-CONTAINING VESICLES) IN SUSPENSION-CULTURED GRAPEVINE CELLS, Phyton, 54(2), 1993, pp. 91-98
Citations number
19
Categorie Soggetti
Plant Sciences
Journal title
Phyton → ACNP
ISSN journal
00319457
Volume
54
Issue
2
Year of publication
1993
Pages
91 - 98
Database
ISI
SICI code
0031-9457(1993)54:2<91:EFNLOA>2.0.ZU;2-A
Abstract
The intracellular localization of anthocyanoplasts (anthocyanin-contai ning vesicles) has been studied in grapevine (Vitis vinifera cv. Gamay ) by light microscope examination of protoplasts and vacuoles obtained from suspension cell cultures. In protoplasts, anthocyanin-containing vesicles of approx. 1-10 mum diameter can be observed in the cytoplas m and are absent in vacuoles isolated by means of a polybase or phosph ate buffer lysis procedure. It is concluded that anthocyanoplasts are located in the cytoplasm of suspension cultured grapevine cells.