INCORPORATION OF LYSINE AND LYSINE DIPEPTIDES INTO ALPHA(S1)-CASEIN BY CA2-INDEPENDENT MICROBIAL TRANSGLUTAMINASE()

Ca2+-independent microbial transglutaminase of a variant of Streptover ticillium mobaraense was compared with Ca2+-dependent guinea pig liver transglutaminase as a tool for incorporating lysine or lysine dipepti des into a food protein, The microbial enzyme was able to incorporate up to ca, 12 mol lysine into a mole of citroconylated alpha(s1)-casein . Twenty two lysine dipeptides could be the substrate for incorporatio n catalyzed by the enzyme, The microbial enzyme showed much higher inc orporation of lysine or lysine dipeptides than the guinea pig liver en zyme, which was attributed to the higher themostability of the microbi al enzyme.