ATTEMPTS OF APPLYING COVALENT AFFINITY-CHROMATOGRAPHY FOR DETERMINATION OF ENVIRONMENTAL EXPOSITION TO HEAVY-METALS BASED ON THE QUANTITATIVE ISOLATION OF METALLOTHIONEIN SPECIFIC PROTEIN FROM HUMAN URINE

The aim of this study was to test a new chromatographic method for the quantitative determination of metallothionein (MT), in order to deter mine the level of exposition to heavy metals. For MTs isolation covale nt affinity chromatography was applied, which is a modern technique of separation with good repeatability and reproducibility, allowing spec ific isolation of the thiolproteins. Fundamentals of indirect determin ation of the contents of metallothionein protein were worked out by es timation of the quantities of metal bound to metallothionein protein a nd adsorbed on covalent affinity gel during a separating process.