The seed oil of Pimpinella acuminata species of the family Umbellifera
e was extracted with chloroform : methanol (2:1) for the separation of
different classes of lipids as hydrocarbons (1.7%), sterol esters (tr
aces), triglycerides (74.1%), free fatty acids (6.6%), 1,3-diglyceride
s (1.6%), 1,2-diglycerides (1.6%), sterols (1.8%), monoglycerides (2.0
%), phosphatidyl ethanolamine (0.8%), phosphatidyl choline (1.2%), lys
e phosphatidyl ethanolamine (0.4%), phosphatidyl inositol (1.2%) and u
nknown (7.0%). The fatty acids as determined by application of gas liq
uid chromatography is C-10-C-22, whereas C-16:0 C-18:0, C-18:1 and C-1
8:2 are predominantly present in all polar and nonpolar lipid classes.