CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A BACTERIAL GLUTATHIONE TRANSFERASE

Authors
FEIL SC WILCE MCJ ROSSJOHN J ALLOCATI N ACETO A DIILIO C PARKER MW
Citation
Sc. Feil et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A BACTERIAL GLUTATHIONE TRANSFERASE, Acta crystallographica. Section D, Biological crystallography, 52, 1996, pp. 189-191
Citations number
30
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
52
Year of publication
1996
Part
1
Pages
189 - 191
Database
ISI
SICI code
0907-4449(1996)52:<189:CAPAOA>2.0.ZU;2-K
Abstract
Crystals of a bacterial glutathione S-transferase from Proteus mirabil is have been grown from polyethylene glycol by the hanging-drop vapour -diffusion method. Successful crystallization required the presence of the substrate glutathione. The crystals belong to the tetragonal spac e group P4 with cell dimensions a = b = 90.9 and c = 117.3 Angstrom. T hey contain between three and six monomers in the asymmetric unit and diffract to beyond 2.3 Angstrom resolution.