M. Panteghini et F. Pagani, BIOLOGICAL VARIATION IN URINARY-EXCRETION OF PYRIDINIUM CROSS-LINKS -RECOMMENDATIONS FOR THE OPTIMUM SPECIMEN, Annals of clinical biochemistry, 33, 1996, pp. 36-42
We assessed the analytical and biological variation of pyridinium cros
slinks in early morning, 2 h fasting, and 24 h urine specimens from 14
healthy adults over a 1 month period. The results were expressed both
in terms of pyridinoline concentration and pyridinoline/creatinine ra
tio. The data obtained were used to select the optimum specimen for cl
inical purposes. We found that: (a) early morning specimens are prefer
red; (b) results should be expressed as pyridinoline/creatinine ratio;
(c) reference intervals should be stratified according to gender; (d)
the necessary analytical imprecision (CV less than or equal to 9%), d
erived from biological variation, is not easily achieved by current me
thods; (e) the difference between serial results from an individual mu
st be > 50% to be statistically significant; and (f) assessment of ris
k for osteoporotic fracture by means of the pyridinium crosslink assay
would, in a significant number of patients, require analysis of multi
ple urine specimens.