DETECTION OF TRIPLET REPEAT SEQUENCES IN YEAST ARTIFICIAL CHROMOSOMESUSING OLIGONUCLEOTIDE PROBES - APPLICATION TO THE SCA1 REGION IN 6P23

In this paper, we describe labeling and hybridization conditions for o ligonucleotide probes that detect human triplet repeat sequences in ye ast artificial chromosomes (YACs). Restriction digests of YACs contain ing the CAG repeat sequence of the SCA1 gene were used as positive con trols. Several hybridization mixtures and temperatures and two differe nt labeling techniques were tested in order to determine optimal condi tions. GAG, CGG, AGG, and ATT repeat sequences were mapped on YACs fro m a contig in 6p23, where SCA1 is located.